Analisis Variasi Genetik Gen L1 HPV (Human Papillomavirus) TIPE 16 Asal Sumatera Barat

Andre Prima Rahmadhana, Marlina Marlina, Akmal Djamaan, Andani Eka Putra, Ifan Aulia Candra

Sari


Variasi nukleotida gen L1 terhadap data terkait di Gene Bank dilakukan dengan menggunakan 5 isolat DNA tipe 16 asal sumbar dimana setelah diamplifikasi hanya 2 yang berhasil di amplifikasi dan dilanjutkan dengan tahap sekuensing yakni sampel 3 dan sampel 4. Analisis dilakukan  menggunakan data sekuensing untuk kemudian dilakukan blast, konstruksi pohon filogenetik. Hasil analisis data sekuensing menunjukkan  sampel 3 memiliki kekerabatan dengan sekuen L1 di Gene Bank KU.9551191.1 dan sampel 4 memiliki kekerabatan dengan sekuen L1 JX313706.1.

 

Kata Kunci: Gen L1, pohon filogenetik, primer BLAST, HPV 16, sekuensing.


Teks Lengkap:

PDF

Referensi


Abdoli, A., H. Soleimanjahi, F. Fotouhi, A. Teimoori, Sh. Pour Beiranvand, dan Z. Kianmehr. 2013. Human Papillomavirus Type16- L1 VLP Production in Insect Cells. Iran J Basic Med Sci, 16: 891-895

Arfiandi. 2016. Analisa Variasi Molekuler dan Filogenetik Gen E7 Isolat HPV 18 dari Penderita Kanker Serviks [Tesis]. Padang. Program Pascasarjana Universitas Andalas. 77 Hal.

Burk, R.D., Z. Chen, dan K. Van Doorslaer. 2009. Human Papillomaviruses: Genetic Basis of Carcinogenicity. Public Health Genomics, 12: 281-290.

Burk, R. D., A. Harari, dan Z. Chen. 2013. Human Papillomavirus Genome Variants. Virology, 445 (0): 232-243.

Chen, Z., M. Schiffman, R. Herrero, R. Desaile, K. Anastos, M. Segondy, V.V. Sahasrabuddhe, P.E. Gravitt, A.W. Hsing, dan R.D. Burk. 2011. Evolution and Taxonomic Classification of Human Papilloma Virus 16 (HPV16)- Related Variant Genomes : HPV 31, HPV 33, HPV 35, HPV 52, HPV 58, and HPV 67. PLoS ONE, 6 (5): e20183.

Chiesa, I,J., M.S. Perez, G.G. Nuñez, dan D.A. Pirola. 2015. Genetic variability nd phylogeny nlysis of partial L1 gene of human papillomavirus variants in Buenos Aires, Argentina. VirusDis. (January-March 2016) 27 (1): 41-47

Doorbar J., N. Egawa, H. Griffin, C. Kranjec, dan I. Murakami. 2016. Human papillomavirus molecular biology and disease association. Rev. Med. Virol, 25: 2-23

Else, E.A., R. Swoyer, Y. Zhang, F.J. Taddeo, J.T. Bryan, J. Lawson, I.V. Hyfte, dan C.C. Roberts. 2011. Comparison of Real Time Multiplex Human Papillomavirus (HPV) PCR Assays with INNo-LiPA HVP Genotyping Extra Assay. Journal of clinical Microbiology, 49 (5): 1907-1912.

Gurgel, A.P.A.D., B.S. Chagas, C.M. do Amaral, K.C.G. Nascimento, L.R.S. Leal, J.D.S. Neto, M.T.C. Muniz, dan A.C. de Freitas. 2015. Prevalence of Human Papillomavirus Variants and Genetic Diversity in the L1 Gene and Long Control Region of HPV 16, HPV 31, and HPV 58 Found in North-East Brazil. BioMed Research International

Jagu, S., K. Kwak, J.T. Schiller, D.R. Lowy, H. Kleanthous, K. Kalnin, C. Wang, H-K. Wang, L.T. Chow, W.K. Huh, K.S. Jaganathan, S.V. Chivukula, dan R.B.S. Roden. 2013. Phylogenic Considerations in Designing a broadly Protective Multimeric L2 Vaccine. Journal of Virology, 87 (11): 6127-6136.

Latief, M., I.A. Rini, G.W. Pradini, G.N.A. Winarno, E. Sahiratmadja, dan H. Susanto. 2018. Phylogenetic Analysis of Human Papillomavirus 16 and 52 L1 Gene from Cervical Cancer in Bandung. The Indonesian Biomedical Journal 10 (1): 40-45

Lestari, V.A., I.A. Rini, G.W. Pradini, E. Sahiratmadja, dan H. Susanto. 2018. Phylogeny of HPV-16 and HPV-18 Multiple Infection of a Patient with Cervical Cancer from Dr. Hasan Sadikin General Hospital, Bandung; A Case Report. The Indonesian Biomedical Journal, 10 (3): 284-289

Liu, Y.,Y. Pan, W. Gao, Y. Ke, dan Z. Lu. 2017. Whole-Genome Analysis of Human Papillomavirus Type 16, 18, and 58 Isolated from Cervical Precancer and Cancer Sample in Chinese Women. Scientific Reports, 7:263

Lorenz, T.C. 2012. Polymerase Chain Reaction: Basic Protocol Plus Trouble shooting and Optimization Strategies. J.Vis. Exp. (63): e3998

Sait, K., R. Turki, A.M. Abuzenadah, O.H. Jiffiri, A. Bohmaidah, dan S.S. Sohrab. 2019. Genetic diversity and phylogenetic analysis of HPV 16 & 18 variants isolated from cervical specimens of women in Saudi Arabia. Saudi Journal of Biomedical Science 26: 317-324

Van Doorslaer, K., dan R. D. Burk. 2010. Evolution of Human Papillomavirus Carcinogenicity. Ads Virus Res, 77: 41-62.

Vestheim, H., S.N. Jarman. 2008. Blocking primers to enhance PCR amplification of rare sequences in mixed samples- a case study on prey DNA in Antartic Krill stomach. Frontiers in Zoology, 5:12

World Health Organization (WHO). 21 August 2015. Vaccine and Disease : Human Papillomavirus (HPV). http://www.who.int/immunization/diseases/hpv/en/ di akses pada 19 Januari 2016




DOI: https://doi.org/10.31869/mi.v20i2.7925

Article Metrics

Sari view : 0 times
PDF - 0 times

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##

INDEXED BY :

   
   


Lembaga Penelitian & Pengabdian Masyarakat (LPPM). Universitas Muhammadiyah Sumatera Barat
Jl. Pasir Kandang No.4, Pasie Nan Tigo, Kec. Koto Tangah, Kota Padang, Sumatera Barat 25586. 
Email : lppmumsb@gmail.com



Kunjungan Sampai Saat ini    Web Analytics Made Easy - StatCounter

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.