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Kloning Genα-Amilase Pendegradasi Butir Pati dariBacillus aquimaris MKSC 6.2 pada Vektor pGEM-T
Sari
α-Amylase (EC 3.2.1.1) is an enzyme which hydrolyzes α-1,4 glicosidic bonds in
polysaccharides such as amylose and amylopectin. α-Amylase is widely applied in industry, such as
starch processing, detergent, and pharmaceutical industries. α-Amylase Bacillus aquimaris MKSC
6.2 can degredate raw starch at low temperature, hence it has a potential economic value as an
alternative enzyme in starch processing. The long term goal of this research is to produce B.
aquimaris α-amylase recombinant, while the short term goals of this research were to amplify the
baqA gene by PCR method and to clone the resulted DNA fragment gene in pGEM-T vector. The α-
amylase gene amplified by PCR using forward primer and reverse primer has a size of 1.5 kb.
Recombinant pGEM-T plasmid containing the 1.5 kb α-amylase fragment has been obtained.
Keywords: raw starch,α-amylase,B. aquimarisMKSC 6.2, recombinant plasmid
Teks Lengkap:
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DOI: https://doi.org/10.33559/mi.v12i3.716
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